Measurement of platelet thrombus formation in patients following severe thermal injury
| Autor: | Ali Asiri, Joshua M. J. Price, Jon Hazeldine, Kirsty C. McGee, Amanda V. Sardeli, Yung-Yi Chen, Jack Sullivan, Naiem S. Moiemen, Paul Harrison |
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| Jazyk: | angličtina |
| Rok vydání: | 2024 |
| Předmět: | |
| Zdroj: | Platelets, Vol 35, Iss 1 (2024) |
| Druh dokumentu: | article |
| ISSN: | 09537104 1369-1635 0953-7104 |
| DOI: | 10.1080/09537104.2024.2420952 |
| Popis: | Severe thermal injury significantly impacts upon hemostasis and is associated with classical changes to the circulating platelet count with a nadir followed by a rebound thrombocytosis at days ~3 and ~15 post-injury, respectively. To date, few studies have assessed platelet function following thermal injury as platelet tests often require large quantities of blood, are not representative of normal platelet pathophysiology, and are usually dependent on a normal platelet count. The purpose of this study was to measure platelet thrombus formation in vitro using a whole blood flow chip-based system following thermal injury and to study how platelet counts may impact upon the measurement. Adult (≥16 years) patients (N = 10) with ≥ 20% total burn surface area (TBSA) burn were recruited within 24 h of injury. Healthy controls (N = 25) were also recruited. Whole blood counts were measured using a hematology analyzer (Sysmex XN-1000). Platelet function was measured using the Total Thrombus-formation Analyzer System (T-TAS) within chips coated with tissue factor and collagen at shear rates of either 600 sec−1 (AR chips) or 1200 sec−1 (HD chips), the latter test being independent of platelet count. We confirmed the classical nadir in platelet counts following severe thermal injury at days 2, 3, 4 (p < 0.0001) and day 5 (p |
| Databáze: | Directory of Open Access Journals |
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