The Cytotoxic Effects of Sio2 Nanoparticles on Human Blood Mononuclear Cells
| Autor: | A Jebali, H Hekmatimoghaddam, s Barzegar, H Fallahzadeh, A Barkhordari |
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| Jazyk: | perština |
| Rok vydání: | 2012 |
| Předmět: | |
| Zdroj: | Majallah-i Dānishgāh-i ’Ulūm-i Pizishkī-i Shahīd Ṣadūqī Yazd, Vol 20, Iss 1, Pp 10-18 (2012) |
| Druh dokumentu: | article |
| ISSN: | 2228-5741 2228-5733 |
| Popis: | Introduction: Regarding the increasing use of silicon dioxide nanoparticles in medical biotechnology and probable side effects and diseases resulting from its usage, this study was performed to assess the toxic effects of different concentrations of SiO2 nanoparticles on human blood mononuclear leukocytes using the MTT assay. Methods: In this laboratory trial study, we prepared suspensions of blood mononuclear cells from 10 young healthy men and also different concentrations of the nanoparticles (1, 10, 100, 500, 1000 and 1500µg/mL). The cells were then incubated with these nanoparticles for 24 hours at 37 °c, and finally the percent of dead cells were measured by MTT assay kit using spectrophotometer reading at 490 nm after 6 and 24 hours of incubation. Positive and negative controls and blanking were applied, too. Results: A significant difference was found in percent of dead cells between the different concentrations of SiO2 nanoparticles and also between the exposed cells and control group (p |
| Databáze: | Directory of Open Access Journals |
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