A useful gene cassette for conditional knock-down of essential genes by targeted promoter replacement in Mycobacteria

Autor:	Pauline Texier, Michèle Coddeville, Patricia Bordes, Pierre Genevaux
Jazyk:	angličtina
Rok vydání:	2018
Předmět:	ClpP Mycobacterium smegmatis promoter replacement recombineering SecA1 tetracycline promoter Biology (General) QH301-705.5
Zdroj:	BioTechniques, Vol 65, Iss 3, Pp 159-162 (2018)
Druh dokumentu:	article
ISSN:	1940-9818 0736-6205
DOI:	10.2144/btn-2018-0074
Popis:	A direct method to study essential genes is to construct conditional knock-down mutants by replacement of their native promoter by an inducible one. In Mycobacteria, replacement of an essential gene promoter with an anhydrotetracycline inducible one was successfully used but required a multi-step approach. In this work, we describe a gene cassette for the engineering of a conditional knock-down mutant, which allows the one-step targeted replacement of mycobacterial promoters by an anhydrotetracycline-inducible promoter. The functionality of this cassette was successfully tested by engineering conditional clpP and SecA1 mutants of Mycobacterium smegmatis.
Databáze:	Directory of Open Access Journals
Externí odkaz:	https://doaj.org/article/3ebce568d0d041499f83b32d35e2179f Zobrazit plný text záznamu View record in DOAJ