Fast Adaptive Optics in optically sectioned fluorescence microscopes for functional neuroimaging

Autor: Harms Fabrice, Mercier Mathias, Gauillaume-Manca Alice, Veilly Cynthia, Levecq Xavier, Bourdieu Laurent, Fragola Alexandra
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: EPJ Web of Conferences, Vol 287, p 03005 (2023)
Druh dokumentu: article
ISSN: 2100-014X
DOI: 10.1051/epjconf/202328703005
Popis: We demonstrate how a novel approach for closed-loop Adaptive Optics (AO) specifically adapted to microscopy enables straightforward integration in Light-Sheet and Multiphoton microscopes, as well as fast aberration correction. We present corresponding experimental setups as well as first demonstrations of the benefits of the correction of sample-induced aberrations in zebrafish and mouse brain tissue, with the ultimate goal to enable high-speed, high-sensitivity functional imaging at large depths.
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