| Autor: |
Jessica Lizbeth Sebastián-Nicolas, Elizabeth Contreras-López, Juan Ramírez-Godínez, Alma Elizabeth Cruz-Guerrero, Gabriela Mariana Rodríguez-Serrano, Javier Añorve-Morga, Judith Jaimez-Ordaz, Araceli Castañeda-Ovando, Emmanuel Pérez-Escalante, Alexis Ayala-Niño, Luis Guillermo González-Olivares |
| Jazyk: |
angličtina |
| Rok vydání: |
2021 |
| Předmět: |
|
| Zdroj: |
Fermentation, Vol 7, Iss 4, p 215 (2021) |
| Druh dokumentu: |
article |
| ISSN: |
2311-5637 |
| DOI: |
10.3390/fermentation7040215 |
| Popis: |
Health benefits of probiotics and production of inhibitors of angiotensin converting enzyme (ACE) released during milk fermentation are well known. That is why in this investigation the proteolytic profile and ACE inhibitory capacity of peptide fractions from protein hydrolysis of milk during fermentation processes was analyzed. Milk fermentation was carried out inoculating 106 CFU of L. rhamnosus GG, S. thermophilus SY-102 and with both bacteria. The proteolytic profile was determined using: TNBS, SDS-PAGE and SEC-HPLC techniques. In vitro ACE inhibition capacity was measured. The pH of 4.5 was reached at 56 h when the milk was fermented with L. rhamnosus, at 12 h with S. thermophillus and at 41 h in the co-culture. Production of free amino groups corresponded with the profile of low molecular weight peptides observed by SDS-PAGE and SEC-HPLC. Co-culture fermentation showed both the highest concentration of low molecular weight peptides and the ACE inhibitory activity (>80%). Results indicated that the combination of lactic cultures could be useful in manufacture of fermented milk with an added value that goes beyond basic nutrition, such as the production of ACE-inhibitory peptides. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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