Inhibitory Effect and Mechanism of Sea-buckthorn Flavonoids on Acrylamide Formation by Using the Simulation System

Autor: Zhenyue TANG, Wenxiao FENG, Mengling LÜ, Yusong ZHANG, Weiran CUI, Hongyu BAI, Meili SHAO
Jazyk: čínština
Rok vydání: 2023
Předmět:
Zdroj: Shipin gongye ke-ji, Vol 44, Iss 21, Pp 111-118 (2023)
Druh dokumentu: article
ISSN: 1002-0306
DOI: 10.13386/j.issn1002-0306.2023010066
Popis: This study was designed to explore the inhibitory effect and mechanism of sea-buckthorn flavonoids on the formation of acrylamide. In order to study the inhibitory effect of sea-buckthorn flavonoids on the formation of acrylamide, the dose-effect relationship of sea-buckthorn flavonoids inhibiting the formation of acrylamide was investigated in the Asn-Glu simulation system. Then the formation/elimination kinetic model and mechanism kinetic model were used to evaluate the products of Maillard reaction by UV absorption spectrum, liquid chromatography-tandem mass spectrometry, and high performance liquid chromatography to explore the inhibitory mechanism of sea-buckthorn flavonoids on the formation of acrylamide. The results confirmed that sea-buckthorn flavonoids could effectively reduce the formation of acrylamide and achieve a maximum reduction rate 62.1%. The formation/elimination kinetic model demonstrated that sea-buckthorn flavonoids significantly reduced the formation rate constants a, tcg of acrylamide (P0.05), indicating that sea-buckthorn flavonoids mainly inhibited the formation stage of acrylamide, and had no effect on the elimination process. The mechanism kinetic model suggested that sea-buckthorn flavonoids actively reduced the production rate of acrylamide k2 and the production rate of melanin k3 (P
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