| Autor: |
Ferdinand Ngale Njume, Adria Razzauti, Miguel Soler, Veronika Perschin, Gholamreza Fazeli, Axelle Bourez, Cedric Delporte, Stephen M. Ghogomu, Philippe Poelvoorde, Simon Pichard, Catherine Birck, Arnaud Poterszman, Jacob Souopgui, Pierre Van Antwerpen, Christian Stigloher, Luc Vanhamme, Patrick Laurent |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
iScience, Vol 25, Iss 11, Pp 105357- (2022) |
| Druh dokumentu: |
article |
| ISSN: |
2589-0042 |
| DOI: |
10.1016/j.isci.2022.105357 |
| Popis: |
Summary: The cuticle of C. elegans is impermeable to chemicals, toxins, and pathogens. However, increased permeability is a desirable phenotype because it facilitates chemical uptake. Surface lipids contribute to the permeability barrier. Here, we identify the lipid transfer protein GMAP-1 as a critical element setting the permeability of the C. elegans cuticle. A gmap-1 deletion mutant increases cuticular permeability to sodium azide, levamisole, Hoechst, and DiI. Expressing GMAP-1 in the hypodermis or transiently in the adults is sufficient to rescue this gmap-1 permeability phenotype. GMAP-1 protein is secreted from the hypodermis to the aqueous fluid filling the space between collagen fibers of the cuticle. In vitro, GMAP-1 protein binds phosphatidylserine and phosphatidylcholine while in vivo, GMAP-1 sets the surface lipid composition and organization. Altogether, our results suggest GMAP-1 secreted by hypodermis shuttles lipids to the surface to form the permeability barrier of C. elegans. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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