Original animal model of lumbar disc degeneration
| Autor: | Najah Elmounedi, Walid Bahloul, Mourad Aoui, Nizar Sahnoun, Zoubaier Ellouz, Hassib Keskes |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | Libyan Journal of Medicine, Vol 18, Iss 1 (2023) |
| Druh dokumentu: | article |
| ISSN: | 19932820 1819-6357 1993-2820 |
| DOI: | 10.1080/19932820.2023.2212481 |
| Popis: | ABSTRACTPurpose: Disc degeneration (DD) is a common cause of low back pain, which represents one of the most widespread public health problems in the world. Therefore, the establishment of a reproducible animal model is indispensable to understand the pathogenic mechanisms of DD and to test new therapeutic strategies. From this perspective, the fundamental objective of this study was to elucidate the effect of ovariectomy in establishing a new animal model of DD in rats.Methods: 36 female Sprague-Dawley rats were divided into four groups of 9 rats: Group 1: Negative control (Sham): Only an abdominal skin incision and sutures were performed. Group 2: Ovariectomy (OVX): Removal of two ovaries through a transverse incision in the middle of the abdomen. Group 3: Puncture (Punct): Puncture of lumbar intervertebral discs (L3/4, L4/5, and L5/6) by a 21 G needle. Group 4: Puncture+ovariectomy (Punct+OVX): Removal of two ovaries and puncture of L3/4, L4/5, and L5/6 discs. The rats were euthanized 1, 3, and 6 weeks post-surgery, and the discs were harvested. Validity was assessed by radiography, histology, and biochemistry (water content).Results: Disc height, water content, and histologic score decreased significantly in the last 3 groups and at all three-time points (P |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
|