Autor: |
Narayanan Shyam-Sundar, Ramakrishnan Ramasubramanian, Sengodan Karthi, Sengottayan Senthil-Nathan, Kanagaraj Muthu-Pandian Chanthini, Haridoss Sivanesh, Vethamonickam Stanley-Raja, Govindaraju Ramkumar, Kilapavoor Raman Narayanan, Shahid Mahboob, Khalid Abdullah Al-Ghanim, Ahmed Abdel-Megeed, Patcharin Krutmuang |
Jazyk: |
angličtina |
Rok vydání: |
2022 |
Předmět: |
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Zdroj: |
Frontiers in Physiology, Vol 13 (2022) |
Druh dokumentu: |
article |
ISSN: |
1664-042X |
DOI: |
10.3389/fphys.2022.900570 |
Popis: |
Spodoptera litura (Fabricius) is an agriculturally significant polyphagous insect pest that has evolved a high level of resistance to conventional insecticides. A dietary assay was used in this work to assess the resilience of field populations of S. litura to λ-cyhalothrin. Analysis of the function and expression of the cytochrome P450 gene was used to test the sensitivity of S. litura larvae to sub-lethal concentrations of the insecticidal plant chemical Precocene 1, both by itself and in combination with λ-cyhalothrin. The activity of esterase enzymes (α and β) was found to decrease 48 h post treatment with Precocene 1. The activity of GST enzyme and cytochrome P450 increased with Precocene 1 treatment post 48 h, however. Expression studies revealed the modulation by Precocene 1 of cytochrome P450 genes, CYP4M16, CYP4M15, CYP4S8V4, CYP4G31, and CYP4L10. While CYP4M16 expression was stimulated the most by the synergistic Precocene 1 + λ–cyhalothrin treatment, expression of CYP4G31 was the most down-regulated by Precocene 1 exposure. Hence, it is evident that λ–cyhalothrin-resistant pest populations are still sensitive to Precocene 1 at a sublethal concentration that is nevertheless capable of hindering their development. Precocene 1 can therefore be considered a potent candidate for the effective management of insecticide-resilient S. litura. |
Databáze: |
Directory of Open Access Journals |
Externí odkaz: |
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