| Autor: |
Juan Du, Junlei Zhang, Lin Wang, Xun Wang, Yaxing Zhao, Jiaoying Lu, Tingmin Fan, Meng Niu, Jie Zhang, Fei Cheng, Jun Li, Qi Zhu, Daoqiang Zhang, Hao Pei, Guang Li, Xingguang Liang, He Huang, Xiaocang Cao, Xinjuan Liu, Wei Shao, Jianpeng Sheng |
| Jazyk: |
angličtina |
| Rok vydání: |
2023 |
| Předmět: |
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| Zdroj: |
Nature Communications, Vol 14, Iss 1, Pp 1-17 (2023) |
| Druh dokumentu: |
article |
| ISSN: |
2041-1723 |
| DOI: |
10.1038/s41467-023-39173-2 |
| Popis: |
Abstract Ulcerative colitis is a chronic inflammatory bowel disorder with cellular heterogeneity. To understand the composition and spatial changes of the ulcerative colitis ecosystem, here we use imaging mass cytometry and single-cell RNA sequencing to depict the single-cell landscape of the human colon ecosystem. We find tissue topological changes featured with macrophage disappearance reaction in the ulcerative colitis region, occurring only for tissue-resident macrophages. Reactive oxygen species levels are higher in the ulcerative colitis region, but reactive oxygen species scavenging enzyme SOD2 is barely detected in resident macrophages, resulting in distinct reactive oxygen species vulnerability for inflammatory macrophages and resident macrophages. Inflammatory macrophages replace resident macrophages and cause a spatial shift of TNF production during ulcerative colitis via a cytokine production network formed with T and B cells. Our study suggests components of a mechanism for the observed macrophage disappearance reaction of resident macrophages, providing mechanistic hints for macrophage disappearance reaction in other inflammation or infection situations. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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