First Detection and Molecular Characterization of Usutu Virus in Culex pipiens Mosquitoes Collected in Romania

Autor: Florian Liviu Prioteasa, Sorin Dinu, Georgiana Victorița Tiron, Ioana Georgeta Stancu, Elena Fălcuță, Cornelia Svetlana Ceianu, Ani Ioana Cotar
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Microorganisms, Vol 11, Iss 3, p 684 (2023)
Druh dokumentu: article
ISSN: 2076-2607
DOI: 10.3390/microorganisms11030684
Popis: Usutu virus (USUV) is an emergent arbovirus in Europe causing mortality in bird populations. Similar to West Nile virus (WNV), USUV is maintained in sylvatic cycles between mosquito vectors and bird reservoirs. Spillover events may result in human neurological infection cases. Apart from indirect evidence provided by a recent serological study in wild birds, the circulation of USUV in Romania was not assessed. We aimed to detect and molecular characterize USUV circulating in mosquito vectors collected in South-Eastern Romania—a well-known WNV endemic region—during four transmission seasons. Mosquitoes were collected from Bucharest metropolitan area and Danube Delta, pooled, and screened by real-time RT-PCR for USUV. Partial genomic sequences were obtained and used for phylogeny. USUV was detected in Culex pipiens s.l. female mosquitoes collected in Bucharest, in 2019. The virus belonged to Europe 2 lineage, sub-lineage EU2-A. Phylogenetic analysis revealed high similarity with isolates infecting mosquito vectors, birds, and humans in Europe starting with 2009, all sharing common origin in Northern Italy. To our knowledge, this is the first study characterizing a strain of USUV circulating in Romania.
Databáze: Directory of Open Access Journals