| Autor: |
Qitong Weng, Fangxiao Hu, Mengyun Zhang, Yong Dong, Cui Lv, Ying Wang, Xiaofei Liu, Jinyong Wang |
| Jazyk: |
angličtina |
| Rok vydání: |
2018 |
| Předmět: |
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| Zdroj: |
Cell Regeneration, Vol 7, Iss 1, Pp 7-15 (2018) |
| Druh dokumentu: |
article |
| ISSN: |
2045-9769 |
| DOI: |
10.1016/j.cr.2018.05.001 |
| Popis: |
Obtaining T cells by reprogramming is one of the major goals in regenerative medicine. Here, we describe a protocol for generating functional T cells from Hoxb5-expressing pro/pre-B cells in vivo. This protocol includes the construction of Hoxb5 recombinant plasmids, retroviral packaging, isolation and viral transduction of pro/pre-B cells, cell transplantation, and phenotypic analysis of induced T cells. The procedure is reproducible and straightforward, providing an approach for generating induced T cells for translational research. Keywords: Hoxb5, Retrovirus, Pro/pre-B cells, T cells |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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