Identification of sex chromosomes in Eremias velox (Lacertidae, Reptilia) using lampbrush chromosome analysis
| Autor: | Artem P. Lisachov, Svetlana A. Galkina, Alsu F. Saifitdinova, Svetlana A. Romanenko, Daria A. Andreyushkova, Vladimir A. Trifonov, Pavel M. Borodin |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: | |
| Zdroj: | Comparative Cytogenetics, Vol 13, Iss 2, Pp 121-132 (2019) |
| Druh dokumentu: | article |
| ISSN: | 1993-078X 1993-0771 |
| DOI: | 10.3897/CompCytogen.v13i2.34116 |
| Popis: | Reptiles are good objects for studying the evolution of sex determination, since they have different sex determination systems in different lineages. Lacertid lizards have been long-known for possessing ZZ/ZW type sex chromosomes. However, due to morphological uniformity of lacertid chromosomes, the Z chromosome has been only putatively cytologically identified. We used lampbrush chromosome (LBC) analysis and FISH with a W-specific probe in Eremias velox (Pallas, 1771) to unequivocally identify the ZW bivalent and investigate its meiotic behavior. The heterochromatic W chromosome is decondensed at the lampbrush stage, indicating active transcription, contrast with the highly condensed condition of the lampbrush W chromosomes in birds. We identified the Z chromosome by its chiasmatic association with the W chromosome as chromosome XIII of the 19 chromosomes in the LBC karyotype. Our findings agree with previous genetic and genomic studies, which suggested that the lacertid Z chromosome should be one of the smaller macrochromosomes. |
| Databáze: | Directory of Open Access Journals |
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