Phosphate limitation as crucial factor to enhance yeast lipid production from short‐chain fatty acids

Autor: Sergio Morales‐Palomo, Elia Tomás‐Pejó, Cristina González‐Fernández
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Microbial Biotechnology, Vol 16, Iss 2, Pp 372-380 (2023)
Druh dokumentu: article
ISSN: 1751-7915
DOI: 10.1111/1751-7915.14197
Popis: Abstract Microbial lipids for chemical synthesis are commonly obtained from sugar‐based substrates which in most cases is not economically viable. As a low‐cost carbon source, short‐chain fatty acids (SCFAs) that can be obtained from food wastes offer an interesting alternative for achieving an affordable lipid production process. In this study, SCFAs were employed to accumulate lipids using Yarrowia lipolytica ACA DC 50109. For this purpose, different amounts of SCFAs, sulfate, phosphate and carbon: phosphate ratios were used in both synthetic and real SCFAs‐rich media. Although sulfate limitation did not increase lipid accumulation, phosphate limitation was proved to be an optimal strategy for increasing lipid content and lipid yields in both synthetic and real media, reaching a lipid productivity up to 8.95 g/L h. Remarkably, the highest lipid yield (0.30 g/g) was achieved under phosphate absence condition (0 g/L). This fact demonstrated the suitability of using low‐phosphate concentrations to boost lipid production from SCFAs.
Databáze: Directory of Open Access Journals
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