Real-time polymerase chain reaction for identification of dog meat in adulterated beef meatball using specific primer targeting on cytochrome-b for halal authentication

Autor:	Abdul Rohman, Ni Wayan Pebriyanti, Sismindari, Anjar Windarsih, Dwiky Ramadhani, Rien Larasati, Henny Yulisa
Jazyk:	angličtina
Rok vydání:	2020
Předmět:	beef meatball dog meat halal authentication real-time pcr adulteration Nutrition. Foods and food supply TX341-641 Food processing and manufacture TP368-456
Zdroj:	International Journal of Food Properties, Vol 23, Iss 1, Pp 2231-2241 (2020)
Druh dokumentu:	article
ISSN:	1094-2912 1532-2386 10942912
DOI:	10.1080/10942912.2020.1844748
Popis:	This research aimed to validate real-time polymerase chain reaction using specific primer targeting on cytochrome-b 33 (cyt-b 33). The primer cyt-b 33 could specifically amplify dog meat DNA and method could detect 0.5 pg dog meat DNA in meatball formulation. The repeatability assay was acceptable with RSD value ≤ 25%. The validated method was further used for detection of meatball products commercially sold in Yogyakarta Indonesia, and results revealed that all samples (n = 14) did not contain dog meat DNA. This research finding supports the implementation of Indonesian Law Number 33 of 2014 in developing method for Halal Product Assurance.
Databáze:	Directory of Open Access Journals
Externí odkaz:	https://doaj.org/article/3ce5ba8ff0ae489eba9c42b7aaff2f0f Zobrazit plný text záznamu View record in DOAJ Plný text ve formátu PDF