Therapeutic Effects of Althaea officinalis L. and Metformin on Estradiol-Induced Polycystic Ovary Syndrome in Rats: Insights into The PI3K/AKT Pathway, Inflammation, and Oxidative Stress
| Autor: | Jiefan Gao, Yue Huang, Yangyang Song, Xiao Huang, Dan Zhang, Zhimin Hou |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2024 |
| Předmět: | |
| Zdroj: | Cell Journal, Vol 26, Iss 8, Pp 473-486 (2024) |
| Druh dokumentu: | article |
| ISSN: | 2228-5806 2228-5814 |
| DOI: | 10.22074/cellj.2024.2026329.1550 |
| Popis: | Objective: Polycystic ovary syndrome (PCOS) is one of the most important causes of infertility, irregular menstrual cycles,and anovulation in women. The current study aimed to investigate the therapeutic effects of Althaea officinalis L. (A.officinale) extract on PCOS in rats.Materials and Methods: In this experimental study, 70 rats in 7 groups (n=10/group) were studied for three weeksas follows; healthy control (HC), patient (PCOS), metformin (PCOS+MET), A. officinale treatment (PCOS+250 and500 mg/kg A. officinale) and synergistic (PCOS+MET+250 and 500 mg/kg A. officinale) groups. Luteinizing hormone(LH), folliclestimulating hormone (FSH), progesterone (P) and testosterone (T) levels as well as inflammatorycytokines were measured. Total antioxidant capacity and lipid peroxidation levels were analyzed in ovarian tissue.The expression of GLUT-4, AKT, PI3K, PTEN genes and Ki-67 was assessed by real-time polymerase chain reaction(PCR) and immunohistochemistry, respectively.Results: A. officinale alone and especially in combination with MET moderated inflammatory and antioxidantparameters compared to the PCOS and MET groups. A. officinale in synergistic groups increased the apoptosis ofgranulosa cells by activating the PI3K/AKT pathway, resulting in a rise in the number of Ki-67 positive cells (P |
| Databáze: | Directory of Open Access Journals |
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