| Autor: |
Emanuela Palomba, Valentina Tirelli, Elisabetta de Alteriis, Palma Parascandola, Carmine Landi, Stefano Mazzoleni, Massimo Sanchez |
| Jazyk: |
angličtina |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
PLoS ONE, Vol 16, Iss 6 (2021) |
| Druh dokumentu: |
article |
| ISSN: |
1932-6203 |
| Popis: |
The yeast Saccharomyces cerevisiae is a reference model system and one of the widely used microorganisms in many biotechnological processes. In industrial yeast applications, combined strategies aim to maximize biomass/product yield, with the fed-batch culture being one of the most frequently used. Flow cytometry (FCM) is widely applied in biotechnological processes and represents a key methodology to monitor cell population dynamics. We propose here an application of FCM in the analysis of yeast cell cycle along the time course of a typical S. cerevisiae fed-batch culture. We used two different dyes, SYTOX Green and SYBR Green, with the aim to better define each stage of cell cycle during S. cerevisiae fed-batch culture. The results provide novel insights in the use of FCM cell cycle analysis for the real-time monitoring of S. cerevisiae bioprocesses. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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