Association of Vitamin E with Rapid Thawing on Goat Semen

Autor: Jurandy Mauro Penitente-Filho, Fabrício Albani Oliveira, Carolina Rodriguez Jimenez, Erly Carrascal, Júlio César Oliveira Dias, Gisele Dias Oliveira, Renata Gomes Silveira, Camila Oliveira Silveira, Ciro Alexandre Alves Torres
Jazyk: angličtina
Rok vydání: 2014
Předmět:
Zdroj: The Scientific World Journal, Vol 2014 (2014)
Druh dokumentu: article
ISSN: 2356-6140
1537-744X
DOI: 10.1155/2014/964172
Popis: The aim of this study was to evaluate the effects of vitamin E associated with rapid thawing on cryopreserved goat semen. Two bucks were used and eight ejaculates per animal were collected using artificial vagina. Semen was diluted with the following treatments: BIOXCELL (control), BIOXCELL + Equex (sodium lauryl sulphate) and BIOXCELL + vitamin E 100 μM. Semen was packaged into 0.25 mL straws and cooled at 5°C for 1 hour. Freezing was performed in liquid nitrogen vapor (−155°C) during 15 minutes. Then, the straws were immersed in liquid nitrogen (−196°C). Straws were thawed at 38°C/60 seconds or at 60°C/7 seconds with immediate sperm analysis. Hypoosmotic swelling test was performed adding a 20 μL aliquot of thawed semen to 1 mL of hypoosmotic solution (100 mOsm·Kg−1) followed by incubation during 60 minutes in water bath (38°C). Vitamin E did not affect any studied parameters (P>0.05). Nevertheless, defrosting rate of 60°C/7 seconds improved sperm membrane functional integrity (P
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