Biotechnological Utilisation of Fusel Oil, A Food Industry By-Product - A Kinetic Model on Enzymatic Esterification of i-Amyl Alcohol and Oleic Acid by Candida antarctica Lipase B

Autor: László Gubicza, Erika Fehér, Katalin Bélafi-Bakó, Nándor Nemestóthy
Jazyk: angličtina
Rok vydání: 2008
Předmět:
Zdroj: Food Technology and Biotechnology, Vol 46, Iss 1, Pp 44-50 (2008)
Druh dokumentu: article
ISSN: 1330-9862
1334-2606
Popis: Fusel oil is a by-product of distilleries, its main component is i-amyl alcohol, which can form ester compounds. Esterification of oleic acid and i-amyl alcohol by Candida antarctica lipase B (Novozym 435 preparation) in n-heptane solvent was studied in this work. Ping-pong bi-bi mechanism (inhibition phenomena taken into account) was applied as a complex kinetic model. The parameters of the model were determined by numerical methods. It was found that four-parameter model fitted well with the experimental results and described properly the enzymatic reaction.
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