Replacing critical point drying with a low-cost chemical drying provides comparable surface image quality of glandular trichomes from leaves of Millingtonia hortensis L. f. in scanning electron micrograph

Autor:	Raktim Bhattacharya, Sulagna Saha, Olga Kostina, Lyudmila Muravnik, Adinpunya Mitra
Jazyk:	angličtina
Rok vydání:	2020
Předmět:	Scanning electron microscope Critical-point drying Chemical drying Hexamethyldisilazane Millingtonia hortensis Glandular trichomes Microscopy QH201-278.5
Zdroj:	Applied Microscopy, Vol 50, Iss 1, Pp 1-6 (2020)
Druh dokumentu:	article
ISSN:	2287-4445
DOI:	10.1186/s42649-020-00035-6
Popis:	Abstract Sample preparation including dehydration and drying of samples is the most intricate part of scanning electron microscopy. Most current sample preparation protocols use critical-point drying with liquid carbon dioxide. Very few studies have reported samples that were dried using chemical reagents. In this study, we used hexamethyldisilazane, a chemical drying reagent, to prepare plant samples. As glandular trichomes are among the most fragile and sensitive surface structures found on plants, we used Millingtonia hortensis leaf samples as our study materials because they contain abundant glandular trichomes. The results obtained using this new method are identical to those produced via critical-point drying.
Databáze:	Directory of Open Access Journals
Externí odkaz:	https://doaj.org/article/3c6d7b9a083d451294d7e11294d58e61 Zobrazit plný text záznamu Full text from SpringerLink View record in DOAJ