Determination of insecticides’ lethal concentrations and metabolic enzyme levels in Triatoma dimidiata

Autor: Arturo Acero-Sandoval, R Patricia Penilla-Navarro, Teresa López-Ordóñez, Mario H Rodríguez, José Genaro Ordóñez-González, Francisco Solís-Santoyo, Américo D Rodríguez
Jazyk: English<br />Spanish; Castilian
Rok vydání: 2020
Předmět:
Zdroj: Salud Pública de México, Vol 62, Iss 4, jul-ago, Pp 402-409 (2020)
Druh dokumentu: article
ISSN: 0036-3634
DOI: 10.21149/10133
Popis: Objective. The feasibility of the use of WHO impregnated paper and biochemical assays to determine lethal concentra­tions (LC50 and LC99) and insecticide metabolic enzyme levels of Triatoma dimidiata. Materials and methods. LC50 and LC99 were calculated with WHO papers impregnated at differ­ent concentrations of malathion, propoxur and deltamethrin; the percentage of insensitive acetylcholinesterase (iAChE); and the levels of esterases, glutathione S-transferases, and monooxygenases in laboratory nymphs of the first stage (5 to 7 days), were undertaken using the WHO biochemical assays. Results. Respectively the LC50 and LC99 μg/cm2 obtained for malathion were 43.83 and 114.38, propoxur 4.71 and 19.29, and deltamethrin 5.80 and 40.46. A 30% of the population had an iAChE, and only a few individuals had high P450 and β-eterase levels. Conclusion. Impregnated papers and bio­chemical tests developed by WHO for other insects, proved to be feasible methods in monitoring insecticide resistance and metabolic enzymes involved in T. dimidiata.
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