Preparation of colloidal gold immunochromatographic test strips for the diagnosis of Toxoplasma gondii

Autor: Yuanxi Shen, Zhaozhe Wang, Jiajing Li, Rui Xu, Rongyi Ji, Jiaojiao Lin, Chuangang Zhu
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Food and Agricultural Immunology, Vol 31, Iss 1, Pp 630-641 (2020)
Druh dokumentu: article
ISSN: 0954-0105
1465-3443
09540105
DOI: 10.1080/09540105.2020.1749569
Popis: As a severely harmful zoonotic disease, toxoplasmosis has complex clinical symptoms with atypical lesions, thus making it difficult for diagnosis in a timely manner. Therefore, developing an appropriate technique to diagnose and prevent toxoplasmosis has become a subject of many studies. In this study, a colloidal gold immunochromatographic test strips for the diagnosis of Toxoplasma gondii was established on the basis of the optimization of antigenic epitopes of GRA1 and GRA7 genes. Western blot and ELISA results showed that expressed rGRA exhibited specifically reaction with Toxoplasma gondii-positive mouse serum. This strip could be used to diagnose the toxoplasmosis of mouse. Moreover, compared with ELISA and PCR, GICA strip exhibited similar sensitivity in the diagnosis of Toxoplasma gondii in pigs. Here, we preliminarily established a diagnostic method for rapidly detecting toxoplasmosis, which may provide technical support for large-scale screening of Toxoplasma gondii.
Databáze: Directory of Open Access Journals
Externí odkaz:
Nepřihlášeným uživatelům se plný text nezobrazuje