Advancing skin model development: A focus on a self-assembled, induced pluripotent stem cell-derived, xeno-free approach

Autor: Marla Dubau, Tarada Tripetchr, Lava Mahmoud, Vivian Kral, Burkhard Kleuser
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: Journal of Tissue Engineering, Vol 15 (2024)
Druh dokumentu: article
ISSN: 2041-7314
20417314
DOI: 10.1177/20417314241291848
Popis: The demand for skin models as alternatives to animal testing has grown due to ethical concerns and the need for accurate substance evaluation. These alternatives, known as New Approach Methodologies (NAMs), are increasingly used for regulatory decisions. Current skin models from primary human cells often rely on bovine collagen, raising ethical issues. This study explores self-assembled skin models (SASM) as a new method, utilizing hair follicle-derived keratinocytes reprogrammed into induced pluripotent stem cells (iPSC) and differentiated into fibroblasts and keratinocytes. The model relies on the ability of fibroblasts to secrete collagen to produce a xeno-free dermal layer and on the differentiation of keratinocytes to create a functional epidermal layer. These layers exhibited confirmed metabolic activity and the capability to withstand test substances. The successful development of SASM underscores the significance of accurate alternatives in dermatological research, providing an ethical and reliable option for substance evaluation and regulatory testing.
Databáze: Directory of Open Access Journals
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