Growth behavior of probiotic microorganisms on levan- and inulin-based fructans

Autor: Joana Charlot Pohlentz, Nicole Gallala, Konrad Kosciow, Marcel Hövels
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Journal of Functional Foods, Vol 99, Iss , Pp 105343- (2022)
Druh dokumentu: article
ISSN: 1756-4646
DOI: 10.1016/j.jff.2022.105343
Popis: Modulation of the gut microbiota by prebiotics is an effective tool to improve host health. Here, the prebiotic properties of inulin- and levan-based fructans were investigated using microorganisms obtained from strain collections and isolated from probiotic pharmaceuticals and yogurts. Utilizing the recently characterized endo-levanase LevB2286, levan-fructooligosaccharides (L-FOS) were produced and applied for dedicated growth studies. Real-time growth experiments in 48-well format revealed that 8 out of 17 strains isolated from probiotic products or yogurts responded to prebiotic treatment. Lactobacillus paracasei strains, several bifidobacteria, and a Saccharomyces cerevisiae isolate metabolized supplied fructans efficiently. Overall inulin-FOS were consumed more rapidly than L-FOS. However, this effect may be attributed to the lower average degree of polymerization and the presence of GFn-FOS in the I-FOS preparation used. Growth experiments with fractionated L-FOS provided valuable insights into the influence of the degree of polymerization on fermentability by probiotic bacteria.
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