Bacteriocin Producing Bacteria Isolated from Turkish Traditional Sausage Samples

Autor: Elif Erkaya, Berna Genc, Sumeyya Akbulut, Gulsah Adiguzel, Mehmet Akif Omeroglu, Hakan Ozkan, Ahmet Adiguzel
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Zdroj: Journal of Pure and Applied Microbiology, Vol 14, Iss 2, Pp 1567-1576 (2020)
Druh dokumentu: article
ISSN: 0973-7510
2581-690X
DOI: 10.22207/JPAM.14.2.55
Popis: In this study, traditional sausage samples from different provinces of Turkey (Gaziantep, Antalya, Erzurum and Kahramanmaras) were obtained and one hundred three isolates were collected. Using the (GTG)5 -PCR genomic fingerprint analysis method, seven of them were observed to be different and conventional tests of these isolates were performed. Molecular identification of two isolates carrying the bacteriocin gene and having antimicrobial activity by agar disc diffusion method was performed by 16S rRNA sequence analysis. As a result, the seven isolates were identified as Aerococcus urinaeequi (EK1), Streptococcus salivarius (EK2), Leuconostoc mesenteroides (EK3), Macrococcus caseolyticus (EK4), Lactococcus garvieae (EK5), Staphylococcus saprophyticus (EK6) and Lactobacillus sakei (EK7). Among these strains, it has been determined that Ln. mesenteroides and L. sakei carried the mecentericin and sacacin genes. When antimicrobial activity against different strains was examined, inhibition formations of Ln. mesenteroides and L. sakei on Enterococcus faecalis, Shigella dysenteriae and Escherichia coli O157: H7 were observed.
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