| Popis: |
In order to clarify the molecular bioinformatics characteristics and tissue expression patterns of the COBRA gene family members of Huperzia serrata, the physicochemical properties, domains, conserved motifs, cis-acting elements, and genes expression of the family members (HsCOBRAs) were analyzed by bioinformatics techniques, based on the full-length transcriptome data of the H. serrata. The results were as follows: (1) A total of 24 HsCOBRAs family members were screened in the full-length transcriptome of H. serrata, including 9 acidic proteins, 11 stabilizing proteins, 5 hydrophobic proteins, 7 proteins with transmembrane structures, and 3 proteins with signal peptides. (2) Subcellular localization was found in the cell wall, chloroplast, nucleus, and cell membrane. (3) Structural analysis revealed that HsCOBRAs had 7 domains and 6 conserved motifs, and partial members had a highly conserved CCVS structure. (4) HsCOBRAs had 45 cis-acting elements such as CAAT-box and TATA-box. (5) HsCOBRA2 had the highest expressions in leaves, spores, stems and gemma. The study results can provide theoretical basis for further research and biological function verification of HsCOBRAs. |
