Utility of paired plasma and drainage fluid mNGS in diagnosing acute intra-abdominal infections with sepsis

Autor:	Jia-yu Mao, Dong-kai Li, Dong Zhang, Qi-wen Yang, Yun Long, Na Cui
Jazyk:	angličtina
Rok vydání:	2024
Předmět:	Sepsis Acute intra-abdominal infection mNGS Plasma and peritoneal drainage Antibiotics Infectious and parasitic diseases RC109-216
Zdroj:	BMC Infectious Diseases, Vol 24, Iss 1, Pp 1-9 (2024)
Druh dokumentu:	article
ISSN:	1471-2334
DOI:	10.1186/s12879-024-09320-1
Popis:	Abstract Background Metagenomic next-generation sequencing (mNGS) has been increasingly applied in sepsis. We aimed to evaluate the diagnostic and therapeutic utility of mNGS of paired plasma and peritoneal drainage (PD) fluid samples in comparison to culture-based microbiological tests (CMTs) among critically ill patients with suspected acute intra-abdominal infections (IAIs). Methods We conducted a prospective study from October 2021 to December 2022 enrolling septic patients with suspected IAIs (n = 111). Pairwise CMTs and mNGS of plasma and PD fluid were sent for pathogen detection. The mNGS group underwent therapeutic regimen adjustment based on mNGS results for better treatment. The microbial community structure, clinical features, antibiotic use and prognoses of the patients were analyzed. Results Higher positivity rates were observed with mNGS versus CMTs for both PD fluid (90.0% vs. 48.3%, p
Databáze:	Directory of Open Access Journals
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