The effect of Cissus quadrangularis Salisb. extract on maturation of rat mesenchymal stem cells
| Autor: | K. Eriani, D. Desriani, Vianti C. Putri, R. Nursanty, S. Mariya, S. Umaratusalihah, I. Ichsan, N. Saidi |
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| Jazyk: | angličtina |
| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | Brazilian Journal of Biology, Vol 83 (2023) |
| Druh dokumentu: | article |
| ISSN: | 1678-4375 1519-6984 |
| DOI: | 10.1590/1519-6984.270335 |
| Popis: | Abstract Degenerative diseases, such as osteoporosis, could be treated by stem cells. The aim of this study was to identify the gene expression of bone marrow mesenchymal stem cells (BM-MSC) derived from Sprague Dawley rats and to assess the effect of Cissus quadrangularis Salisb. extract on their maturation into bone cells. The BM-MSC were divided into three groups: (a) BM-MSCs + osteoblast cell growth basal medium as the positive control; (b) BM-MSCs + Dulbecco’s modified eagle’s medium (DMEM) + 0.3 mg/mL methanol extract of C. quadrangularis as methanol group; and (c) BM-MSC + DMEM + 0.3 mg/mL ethyl acetate extract of C. quadrangularis as ethyl acetate group. A relative quantification approach using was used to analyze the expression of the alp (alkaline phosphatase) gene, with the beta-actin gene was used to normalize the expression of the alp gene. The intra-assay variation was calculated to validate the RT-qPCR data. Our study found that the intra-assay variation value was acceptable, with most of the coefficients of variability (CV) value |
| Databáze: | Directory of Open Access Journals |
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