Flow cytometry-based viability staining: an at-line tool for bioprocess monitoring of Sulfolobus acidocaldarius

Autor: Kerstin Rastädter, Andrea Tramontano, David J. Wurm, Oliver Spadiut, Julian Quehenberger
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: AMB Express, Vol 12, Iss 1, Pp 1-9 (2022)
Druh dokumentu: article
ISSN: 2191-0855
DOI: 10.1186/s13568-022-01447-1
Popis: Key points Development of a flow cytometry (FCM) method for viability determination of S. acidocaldarius using the fluorescent dyes fluorescein diacetate and concanavalin A conjugated with rhodamine. Applicability of the developed method was shown via viability monitoring during a continuous cultivation with triggered pH shifts. A logarithmic trend was observed between the developed FCM method and the state-of-the-art method, plating assay.
Databáze: Directory of Open Access Journals
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