| Autor: |
Ana Paula de Souza Kussler, Ivan Cunha Bustamante Filho, Elisa Negri, Edison Capp, Helena von Eye Corleta |
| Jazyk: |
English<br />Portuguese |
| Rok vydání: |
2024 |
| Předmět: |
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| Zdroj: |
Revista Brasileira de Ginecologia e Obstetrícia, Vol 46 (2024) |
| Druh dokumentu: |
article |
| ISSN: |
0100-7203 |
| DOI: |
10.61622/rbgo/2024rbgo36 |
| Popis: |
Abstract Objective: Seminal cryopreservation causes significant damage to the sperm; therefore, different methods of cryopreservation have been studied. The aim of the study was to compare the effects of density gradient processing and washing/centrifugation with seminal plasma removal for cryopreservation in semen parameters. Methods: Seminal samples of 26 normozoospermic patients were divided into 3 parts: with seminal plasma; after washing/centrifugation; and after selection through density gradient. The samples were cryopreserved for at least two weeks. Motility, sperm count, morphology and viability were evaluated before cryopreservation and after thawing. Results: Density gradient processing selected motile and viable sperm with normal morphology in fresh samples (p |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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