16S rRNA amplicon sequencing dataset for conventionalized and conventionally raised zebrafish larvae

Autor: Daniel J. Davis, Elizabeth C. Bryda, Catherine H. Gillespie, Aaron C. Ericsson
Jazyk: angličtina
Rok vydání: 2016
Předmět:
Zdroj: Data in Brief, Vol 8, Iss , Pp 938-943 (2016)
Druh dokumentu: article
ISSN: 2352-3409
DOI: 10.1016/j.dib.2016.06.057
Popis: Data presented here contains metagenomic analysis regarding the sequential conventionalization of germ-free zebrafish embryos. Zebrafish embryos that underwent a germ-free sterilization process immediately after fertilization were promptly exposed to and raised to larval stage in conventional fish water. At 6 days postfertilization (dpf), these “conventionalized” larvae were compared to zebrafish larvae that were raised in conventional fish water never undergoing the initial sterilization process. Bacterial 16S rRNA amplicon sequencing was performed on DNA isolated from homogenates of the larvae revealing distinct microbiota variations between the two groups. The dataset described here is also related to the research article entitled “Microbial modulation of behavior and stress responses in zebrafish larvae” (Davis et al., 2016) [1]. Keywords: Microbiome, Microbiota, Zebrafish larvae, 16S rRNA sequencing, Gnotobiotic
Databáze: Directory of Open Access Journals