| Popis: |
The ethyl acetate fraction (EAF) of Stelechocarpus burahol leaves is known to contain flavonoids and has antihyperuricemic activity. Its low solubility and large particle size (PS) can reduce its activity. The polyphenolic compounds are also susceptible to oxidation, therefore protection is needed by forming encapsulation. The research focus was to synthesize, characterize, and optimize EAF nanoparticles (EAF-NPs) which were made using the ionic gelation method, then a study was carried out on the influencing factors, and their interactions on the response of PS, polydispersity index (PdI), zeta potential (ZP), and entrapment efficiency (EE). The EAF was obtained from leaves extract by liquid–liquid extraction. Composition of chitosan (CS) and sodium-tripolyphosphate (NaTPP) was determined using D-Optimal design. Experimental results of 13 compositions were obtained values range of PS 204.5 ± 0.72–405.4 ± 12.23 nm, PdI 0.494 ± 0.05–0.634 ± 0.02, ZP −37.7 ± 2.78–−56.1 ± 3.41 mV, and EE 84.76% ±0.03%–92.40% ±0.07%. D-optimal design analysis produced the optimized NPs composition at 10 mL of 0.3% EAF and 90 mL of CS: NaTPP (4.73:0.77). Experimental result of optimized NPs was obtained value of PS 310.6±28.34 nm, PdI 0.377 ± 0.01, ZP −37.2 ± 0.06 mV, and EE 88.93% ±0.02%, which as shown a good characteristic. |
