Alanine-specific appetite in slow growing chickens is associated with impaired glucose transport and TCA cycle

Autor: Shahram Niknafs, Marina R. S. Fortes, Sungbo Cho, John L. Black, Eugeni Roura
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: BMC Genomics, Vol 23, Iss 1, Pp 1-16 (2022)
Druh dokumentu: article
ISSN: 1471-2164
DOI: 10.1186/s12864-022-08625-2
Popis: Abstract Background The rate of protein accretion and growth affect amino acid requirements in young animals. Differences in amino acid metabolism contribute to individual variations in growth rate. This study aimed at determining how amino acid needs may change with growth rates in broiler chickens. Experiment 1 consisted of testing amino acid choices in two chicken groups with extreme growth rates (the slowest –SG- or fastest –FG- growing birds in a flock). Essential (EAA) (methionine, lysine and threonine) or non-essential (NEAA) (alanine, aspartic acid and asparagine) amino acids were added to a standard control feed (13.2 MJ/kg; 21.6% crude protein). The chickens were offered simultaneous access to the control feed and a feed supplemented with one of the two amino acid mixes added at 73% above standard dietary levels. Experiment 2 consisted of the selection of the bottom 5 SG and top 5 FG chickens from a flock of 580 to study differences in amino acid metabolism using the proventriculus representing gut sensing mechanism. In this experiment, transcriptomic, proteomic, and genomic analyses were used to compare the two groups of chickens. Results SG preferred NEAA, while they rejected EAA supplemented feeds (P 0.05). Transcriptomic and proteomic analyses identified 909 differentially expressed genes and 146 differentially abundant proteins associated with differences in growth rate (P
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