| Autor: |
Leticia Mendes Pupio Maia, Aloysio de Mello Figueiredo Cerqueira, Daniel de Barros Macieira, Aline Moreira de Souza, Namir Santos Moreira, Adrianna Vieira da Silva, Joanne Belle Messick, Renata Fernandes Ferreira, Nádia Regina Pereira Almosny |
| Jazyk: |
English<br />Spanish; Castilian<br />Portuguese |
| Předmět: |
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| Zdroj: |
Revista Brasileira de Parasitologia Veterinária, Vol 22, Iss 2, Pp 289-291 |
| Druh dokumentu: |
article |
| ISSN: |
1984-2961 |
| DOI: |
10.1590/S1984-29612013000200049 |
| Popis: |
This article describes the first detection of Cytauxzoon felis, using molecular techniques, in a naturally infected domestic cat from Brazil, South America. Coinfection with 'CandidatusMycoplasma haemominutum' was also found. The molecular identification of the piroplasmid species was performed by Polymerase Chain Reaction (PCR) and sequencing analysis. A 284 pb fragment of the gene encoding the 18S ribosomal RNA region was amplified and showed 99% identity with other C. felis strains from North America. In addition, PCR-RFLP (restriction fragment length polymorphism) analysis, which amplifies a 595 bp fragment of the gene encoding 16S ribosomal RNA of some bacterial species, identified the co-infecting species as 'Candidatus M. haemominutum'. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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