CD9 Expression by Human Granulosa Cells and Platelets as a Predictor of Fertilization Success during IVF

Autor: Carolyn R. Jaslow, Kyle S. Patterson, Shila Cholera, Lisa K. Jennings, Raymond W. Ke, William H. Kutteh
Jazyk: angličtina
Rok vydání: 2010
Předmět:
Zdroj: Obstetrics and Gynecology International, Vol 2010 (2010)
Druh dokumentu: article
ISSN: 1687-9589
1687-9597
DOI: 10.1155/2010/192461
Popis: Objective. To determine whether CD9 expression on human granulosa cells (GCs) and platelets could predict the success of conventional fertilization of human oocytes during in vitro fertilization (IVF). Methods. Thirty women undergoing IVF for nonmale factor infertility participated. Platelets from venous blood and GCs separated from retrieved oocytes were prepared for immunofluorescence. Flow cytometry quantified the percent of GCs expressing CD9, and CD9 surface density on GCs and platelets. Fertilization rate was determined for the total number of oocytes, and the number of mature oocytes per patient. Correlations tested for significant relationships (P
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