https://www.tci-thaijo.org/index.php/easr/article/view/152997/128523

Autor: Bhumin Than-ardna, Hiroshi Tamura, Tetsuya Furuike
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: Engineering and Applied Science Research, Vol 46, Iss 1, Pp 64-71 (2019)
Druh dokumentu: article
ISSN: 2539-6161
2539-6218
DOI: 10.14456/easr.2019.8
Popis: In this study, a process for the removal of proteins from skim natural rubber latex (SNRL) was developed. This process involves a one-step incubation with urea in the presence of a surfactant and an organic solvent, followed by leaching. After 1 h of incubation in 0.1 wt% urea, 1 wt% sodium dodecyl sulfate (SDS) and 1% ethanol, the extractable protein (EP) content was lower than thatof a deproteinized SNRL (D-SNRL) prepared via a single step. To further reduce the protein content, leaching with SDS coupled with 1–3 centrifugation cycles was performed. The EP content of D-SNRL with an additional leaching step and 3 centrifugation cycles under the optimum conditions was found to be 0.179 mg/g. Additionally, the nitrogen content, as determined by the Kjeldahl method, confirmed that the protein content was decreased to 0.053%, which was lower than those of the SNRL and D-SNRL samples processed in a single step. Fourier transform infrared spectra confirmed that the protein contents of thesesamples decreased with an increasing number of leaching steps. Thermogravimetric analysis showed quite similar thermal stability for the product of eachdeproteinization process. The tensile strength and elongation at breakageof the rubber film decreased after protein removal. Scanning electron microscopyrevealed that all rubber films had quite smooth surfaces. This deproteinization method with an additional leaching step allowed for highly efficient protein reduction of up to 96%. This method is expected to improve the quality of skim latex products for several biomedical applications.
Databáze: Directory of Open Access Journals
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