Noninvasive assessment and quantification of tumor vascularization using [18F]FDG-PET/CT and CE-CT in a tumor model with modifiable angiogenesis—an animal experimental prospective cohort study

Autor: Martin Mirus, Sergey V. Tokalov, Andrij Abramyuk, Jessica Heinold, Vincent Prochnow, Klaus Zöphel, Jörg Kotzerke, Nasreddin Abolmaali
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: EJNMMI Research, Vol 9, Iss 1, Pp 1-17 (2019)
Druh dokumentu: article
ISSN: 2191-219X
DOI: 10.1186/s13550-019-0502-0
Popis: Abstract Background This study investigated the noninvasive assessment of tumor vascularization with clinical F-18-fluorodeoxyglucose positron emission tomography/computed tomography and contrast-enhanced computed tomography ([18F]FDG-PET/CT and CE-CT) in experimental human xenograft tumors with modifiable vascularization and compared results to histology. Tumor xenografts with modifiable vascularization were established in 71 athymic nude rats by subcutaneous transplantation of human non-small-cell lung cancer (NSCLC) cells. Four different groups were transplanted with two different tumor cell lines (either A549 or H1299) alone or tumors co-transplanted with rat glomerular endothelial (RGE) cells, the latter to increase vascularization. Tumors were assessed noninvasively by [18F]FDG PET/CT and contrast-enhanced CT (CE-CT) using clinical scanners. This was followed by histological examinations evaluating tumor vasculature (CD-31 and intravascular fluorescent beads). Results In both tumor lines (A549 and H1299), co-transplantation of RGE cells resulted in faster growth rates [maximal tumor diameter of 20 mm after 22 (± 1.2) as compared to 45 (± 1.8) days, p
Databáze: Directory of Open Access Journals
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