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Due to its low-calorie property, Stevia rebaudianais being promoted as an alterna-tive sweetener for diabetic and obese patients. The steady demand in the market for high-quality stevia extracts presents a challenge for the enhanced production of ste-viol glycosides that are safe for human consumption. This study characterized the structure and content of the gene involved in the production of UGT74G1protein for Steviarebaudianaaccession MS007 through in silicoanalysis using a transcrip-tome dataset of stevia MS007. Homologous search using BLASTp shows high sim-ilarity to Q6VAA6 RecName: Full=UDP-glycosyltransferase 74G1 (S. rebaudiana) as the top hit sequence. InterPro family and domain protein motif search revealed UDP-glucuronosyl/UDP-glucosyltransferase (IPR002213) and UDP-glycosyltrans-ferase family, conserved site (IPR035595). The phylogenetic tree construction was done by selecting 14 out of 102 protein sequences from BLASTp search. The phy-logenetic analysis revealed a high value of bootstrapping, which was 100, indicating the high similarity betweenUGT74G1 (Q6VAA6.1 and Cluster-31069.45201) in S. rebaudiana. ProtParam ExPASy, PSIPRED,and Phyre2 computed the primary, sec-ondary, and tertiary structures for UGT74G1protein. The UGT74G1predicted ter-tiary structure scored 100.0% confidence by the single highest scoring template and coverage of 96%. The model has dimensions (Å) of X: 57.609, Y: 70.386, and Z: 58.351. Outcomes of this research will help enhance understandingUDP-glycosyl-transferase 74G1 (S. rebaudianaMS007) characteristicsand enhance target identi-fication processes to improve understanding of protein-protein interaction in S. re-baudianaMS007. |
