| Autor: |
ZHOU Fan-qi, FENG Wen-xin, TAN Pu-wen, MA Yan-ni, WANG Dong, YU Jia |
| Jazyk: |
čínština |
| Rok vydání: |
2020 |
| Předmět: |
|
| Zdroj: |
Jichu yixue yu linchuang, Vol 40, Iss 6, Pp 765-770 (2020) |
| Druh dokumentu: |
article |
| ISSN: |
1001-6325 |
| Popis: |
Objective To investigate the localization of RNA in cytoplasm and nucleus of human embryonic stem cells (hESCs). Methods After separating cytoplasm and nucleus of hESCs, RNAs were extracted for sequencing, and the localization characteristics of RNAs were analyzed by bioinformatics. RT-qPCR was used to verify the localization of these RNAs. Results More RNA types were found in the cytoplasm than nucleus, as well as specifically locational RNAs (2 952 in the cytoplasm and 634 in the nucleus, respectively). Conclusions The localization map of RNA in cytoplasm and nucleus of hESCs is described,and the localization of many RNAs is verified. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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