Characterization of trypsin-like enzymes from the midgut of Morimus funereus (Coleoptera: Cerambycidae) larvae

Autor: Lončar N., Božić Nataša, Nenadović Vera, Ivanović Jelisaveta, Vujčić Z.
Jazyk: angličtina
Rok vydání: 2009
Předmět:
Zdroj: Archives of Biological Sciences, Vol 61, Iss 4, Pp 713-718 (2009)
Druh dokumentu: article
ISSN: 0354-4664
DOI: 10.2298/ABS0904713L
Popis: The pH along the midgut of M. funereus larvae had different values, being acidic in the anterior section and basic in the middle and posterior sections. Elastase- and chymotrypsin-like activities were highest in the middle, low in the anterior, and negligible in the posterior section of the midgut. Trypsin-like activities were detected along the whole midgut, with more than 90% of activity in the anterior section. The level of elastase- and chymotrypsin-like activity was very low compared to trypsin-like activity. In the anterior section of the midgut, two isoforms of trypsin-like enzymes were found, both being basic and almost completely inhibited by benzamidine.
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