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Amelework Eshetu Melka,1 Eyasu Makonnen,1 Asfaw Debella,2 Netsanet Fekadu,2 Bekesho Geleta2 1Department of Pharmacology, School of Medicine, College of Health Sciences, Addis Ababa University, Addis Ababa, Ethiopia; 2Directorate of Traditional and Modern Medicine Research, Ethiopian Public Health Institute, Addis Ababa, Ethiopia Abstract: The present study was undertaken to investigate the diuretic activity and acute toxicity profile of the crude aqueous extract and solvent fraction of the leaves of Thymus serrulatus in saline-loaded Swiss albino mice. Mice of either sex were divided into six groups (five animals in each group). The control group received normal saline (25 mL/kg), while the reference group received hydrochlorothiazide (10 mg/kg). Group III to Group VI received the test substances at dose levels of 125, 250, 500, and 1,000 mg/kg orally, respectively. At the end of the fifth hour, urine was collected, and total volume of urine excreted by each animal was recorded. Concentrations of urinary Na+ and K+ were determined, and the Na+/K+ ratio was calculated to make comparison among the groups. The acute toxicity of the most active fraction was also evaluated. The findings demonstrated that the crude aqueous extract of T. serrulatus leaves showed significant diuretic (P |
