Field trial: disinfection of contaminated anesthetic masks for piglets

Autor: L. Friedrich, E. Winner, H. Härtel, S. Gumbert, S. Zöls, M. Ritzmann, M. Beisl, K. Kempf, A. von Altrock, N. Kemper, J. Schulz
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Porcine Health Management, Vol 9, Iss 1, Pp 1-8 (2023)
Druh dokumentu: article
ISSN: 2055-5660
DOI: 10.1186/s40813-023-00321-1
Popis: Abstract This paper aimed to assess the success of cleaning and disinfection on microbiological contamination of anesthetic masks, which were used for automated isoflurane anesthesia for surgical castration of male piglets. Data collection took place on 11 farms in Southern Germany between September 2020 and June 2022. Each farm was visited three times (one farm having two different anesthesia devices was visited six times), and microbiological assessments took place at four sample points (SP): after unpacking the masks (SP0), after disinfection before anesthesia (SP1), after anesthesia of all piglets to be castrated in this run (SP2), and after disinfection after anesthesia (SP3). The microbiological assessment included the determination of total bacteria count, total count of hemolytic and non-hemolytic mesophilic aerotolerant bacteria and a qualitative detection of indicator bacteria Escherichia (E.) coli, extended-spectrum beta-lactamase-producing E. coli (ESBL) and methicillin-resistant Staphylococcus aureus (MRSA). For analysis, a generalized linear mixed model was applied using farms and farm visits as random effects and sampling points nested in farm visits as fixed effect. The fixed effect was highly significant for all three variables (total bacteria count, total count of hemolytic and non-hemolytic mesophilic aerotolerant bacteria) (p
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