Proteomics analysis of tissue samples from patients with squamous cell carcinoma of the penis and positive to human papillomavirus
| Autor: | Leandro Koifman, Paulo Ornellas, Antonio Augusto Ornellas, Denise de Abreu Pereira, Benedeta Russolina Zingali, Silvia Maria Baeta Cavalcanti, Larissa Alves Afonso, Vanessa Sandim, Gilda Alves |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: | |
| Zdroj: | International Brazilian Journal of Urology, Vol 41, Iss 4, Pp 642-654 (2015) |
| Druh dokumentu: | article |
| ISSN: | 1677-6119 1677-5538 |
| DOI: | 10.1590/S1677-5538.IBJU.2014.0051 |
| Popis: | ABSTRACTPurpose:The aim of this study was to identify possible protein biomarkers and/or candidates for therapeutic targets in tissues of patients with SCCP, infected by HPV, applying one dimensional electrophoresis (1DE), followed by direct mass spectrometry (MS) analysis.Materials and Methods:Tissues from 10 HPV positive patients with SCCP and from 10 patients with HPV negative non-tumorous penile foreskins were analyzed applying 1D electrophoresis, followed by analysis with direct mass spectrometry (MS).Results:Sixty-three different proteins were identified in the first group and 50 in the second group. Recognition was possible for 28 proteins exclusively detected in Group 1 and 21 proteins presented only in Group 2.Conclusion:Some proteins in the first group are directly involved in the development of other types of cancer, and therefore, suitable for analysis. Complement C3 protein is a strong candidate for evaluating SCCP patients. |
| Databáze: | Directory of Open Access Journals |
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