| Autor: |
Yang Sing Leong, Mohd Hadri Hafiz Mokhtar, Mohd Saiful Dzulkefly Zan, Norhana Arsad, Farinawati Yazid, Ahmad Ashrif A. Bakar |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
|
| Zdroj: |
Alexandria Engineering Journal, Vol 97, Iss , Pp 14-24 (2024) |
| Druh dokumentu: |
article |
| ISSN: |
1110-0168 |
| DOI: |
10.1016/j.aej.2024.04.009 |
| Popis: |
Optical imaging is crucial for cell examination, but its instruments' bulky optics limit their flexibility, and a single wavelength provides limited sample information. This paper presents a fibre cantilever-based endoscopic scanner for multiwavelength reflectance confocal microscopy. The scanner utilises the dual-core fibre cantilever to produce Lissajous scans while accommodating two distinct wavelengths. Coloured surfaces were imaged at wavelengths of 520 nm, 635 nm and 850 nm, and verified with a colour chart and reflectance spectroscopy. Blue ink-stained plant stem cross-section samples were also imaged and compared. Furthermore, the scanner performed structure morphology imaging on cellular samples. The 520 nm and 635 nm wavelengths create low- and high-contrast images respectively, while the 850 nm wavelength delivers a sharper image while also capturing the surface profile of the target sample. The findings demonstrated the endoscopic scanner’s capability to extract information based on different wavelengths, providing a more diverse view of the sample and enhancing the surface profile. Using 635 nm and 850 nm, the reconstructed images have a contrast enhancement of 67.9% and 90.8% respectively from 520 nm. The smallest feature size measured on the cellular sample is 5 µm. The endoscopic scanner has the potential to study microfluidic and cell morphology. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
|
