| Autor: |
Andrew J. Percy, Jessica Tamura-Wells, Juan Pablo Albar, Kerman Aloria, Ardeshir Amirkhani, Gabriel D.T. Araujo, Jesus M. Arizmendi, Francisco J. Blanco, Francesc Canals, Jin-Young Cho, Núria Colomé-Calls, Fernando J. Corrales, Gilberto Domont, Guadalupe Espadas, Patricia Fernandez-Puente, Concha Gil, Paul A. Haynes, Maria Luisa Hernáez, Jin Young Kim, Arthur Kopylov, Miguel Marcilla, Mathew J. McKay, Mehdi Mirzaei, Mark P. Molloy, Leanne B. Ohlund, Young-Ki Paik, Alberto Paradela, Mark Raftery, Eduard Sabidó, Lekha Sleno, Daniel Wilffert, Justina C. Wolters, Jong Shin Yoo, Victor Zgoda, Carol E. Parker, Christoph H. Borchers |
| Jazyk: |
angličtina |
| Rok vydání: |
2015 |
| Předmět: |
|
| Zdroj: |
EuPA Open Proteomics, Vol 8, Iss C, Pp 6-15 (2015) |
| Druh dokumentu: |
article |
| ISSN: |
2212-9685 |
| DOI: |
10.1016/j.euprot.2015.06.001 |
| Popis: |
The reproducibility of plasma protein quantitation between laboratories and between instrument types was examined in a large-scale international study involving 16 laboratories and 19 LC–MS/MS platforms, using two kits designed to evaluate instrument performance and one kit designed to evaluate the entire bottom-up workflow. There was little effect of instrument type on the quality of the results, demonstrating the robustness of LC/MRM-MS with isotopically labeled standards. Technician skill was a factor, as errors in sample preparation and sub-optimal LC–MS performance were evident. This highlights the importance of proper training and routine quality control before quantitation is done on patient samples. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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