MULTI DRUG RESISTANCE PROFILE AND ANTIBIOFILM ACTIVITY OF GARLIC OIL ON STAPHYLOCOCCUS AUREUS ISOLATED FROM MILK OF CATTLE SUFFERING WITH MASTITIS
| Autor: | Durgesh Mishra, Arpita Shrivastav, Neeraj Shrivastava, Nitesh Kumar, Swatantra Kumar Singh, Rajeev Ranjan, Namrata Upadhyay |
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| Jazyk: | angličtina |
| Rok vydání: | 2024 |
| Předmět: | |
| Zdroj: | Exploratory Animal and Medical Research, Vol 14, Iss 1, Pp 28-36 (2024) |
| Druh dokumentu: | article |
| ISSN: | 2277-470X 2319-247X |
| DOI: | 10.52635/eamr/14.1.28-36 |
| Popis: | The present study was undertaken on 164 bovine milk samples (cow = 113 and Buffalo = 51) collected from various dairy farms in and around Rewa (Madhya Pradesh, India) and only 24 samples (6.83%) were found to be positive for mastitis when screened through California Mastitis Test (CMT). The prevalence of subclinical mastitis in cattle was 21%. All 17 isolates were phenotypically characterized by mannitol fermentation, biochemical tests (acetoin production by Voges-Proskauer test), beta-galactosidase test susceptibility to novobiocin (5 µ µµg disk), resistance to polymyxin B (300 µg disk). We also observed the biofilm formation ability of all the Staphylococcus aureus strains (n=17) by Congo Red Agar (CRA), Microtiter plate (using crystal violet), and light microscopy method, 90% sensitivity was seen through microtiter plate method. Antibiofilm activity of garlic oil was undertaken on positive isolates (3%, 2%, and 1% concentration) along with positive and negative control in every microtiter plate assay. Maximum inhibition was observed at 3% concentration with O.D values ranging from 0.021±0.007 to 0.291 ± 0.005 in all the samples with percent inhibition (50 to 60%). Multi-drug resistance profiles of biofilm-producing isolates were also undertaken against polymyxin, novobiocin, tetracyclines, cotrimoxazole, clindamycin, cefoxitin, and cefoperazone. Eighty-two (82) percent of isolates showed resistance against polymyxin, 52% against novobiocin and erythromycin, and all the isolates showed 100% sensitivity against tetracyclines and cefoxitin. |
| Databáze: | Directory of Open Access Journals |
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