| Autor: |
Alejandro Vallejo, Pilar Vizcarra, Adrián Martín-Hondarza, Sandra Gómez-Maldonado, Johannes Haemmerle, Héctor Velasco, José L. Casado |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
Frontiers in Microbiology, Vol 13 (2022) |
| Druh dokumentu: |
article |
| ISSN: |
1664-302X |
| DOI: |
10.3389/fmicb.2022.1002748 |
| Popis: |
PurposeTo analyze the impact of SARS-COV-2-specific memory B cells (MBC) on the immune response after two doses of mRNA-based Comirnaty COVID-19 vaccine in seronegative health care workers. This study is seeking a rationale for boosting vaccines.MethodsLongitudinal study including 31 seronegative health care workers with undetectable specific MBCs (IgG−MBC− group), 24 seronegative with detectable specific MBCs (IgG−MBC+ group), and 24 seropositive with detectable specific MBCs (IgG+MBC+ group). The level of antibodies that inhibit ACE2-RBD interaction, and anti-Spike IgG, IgA, and IgM antibodies was quantified by ELISA. In addition, specific memory B and T cells were quantified by flow cytometry.ResultsThe level of specific MBCs, and isotypes, in the IgG−MBC− group was lower compared to that found in IgG−MBC+ (p = 0.0001) and IgG+MBC+ (p |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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