Autor: |
Jure Acimovic, Anita Lövgren-Sandblom, Maria Olin, Zeina Ali, Maura Heverin, Rebecca Schüle, Ludger Schöls, Björn Fischler, Peter Fickert, Michael Trauner, Ingemar Björkhem |
Jazyk: |
angličtina |
Rok vydání: |
2013 |
Předmět: |
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Zdroj: |
PLoS ONE, Vol 8, Iss 7, p e68031 (2013) |
Druh dokumentu: |
article |
ISSN: |
1932-6203 |
DOI: |
10.1371/journal.pone.0068031 |
Popis: |
24S- and 27-hydroxycholesterol (24OHC and 27OHC) are potent regulators of different biochemical systems in vitro and are the major circulating oxysterols. A small fraction of these oxysterols has been reported to be sulphated but there are no detailed studies. We considered the possibility that sulphatation is a protective mechanism preventing accumulation of free oxysterols. Using an accurate assay we found the sulphated fraction of 24OHC and 27OHC in circulation of adults to be less than 15% of total. In two patients with a mutation in CYP7B1 and markedly increased levels of 27OHC the sulphated fraction was 8% and 10% respectively. Infants with severe neonatal cholestasis had however markedly increased sulphate fraction of the above oxysterols. In untreated mice the degree of sulphatation of 24OHC and 27OHC in serum varied between 0 and 16%. Similar degree of sulphatation was found in two mouse models with markedly increased levels of 27OHC and 24OHC respectively. Bile duct ligated mice had higher levels of oxysterols than sham-operated controls but the sulphate fraction was not increased. We conclude that a primary increase in the levels of the oxysterols due to increased synthesis or reduced metabolism in adults and mice does not induce increased sulphatation. |
Databáze: |
Directory of Open Access Journals |
Externí odkaz: |
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