Protocols for accelerated production and purification of collagen scaffold and atelocollagen from animal tissues
| Autor: | Dar-Jen Hsieh, Periasamy Srinivasan |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | BioTechniques, Vol 69, Iss 3, Pp 220-225 (2020) |
| Druh dokumentu: | article |
| ISSN: | 1940-9818 0736-6205 |
| DOI: | 10.2144/btn-2020-0070 |
| Popis: | Traditional purification of atelocollagen involves a harsh extraction process with environment-polluting chemicals and costly and/or time-consuming procedures which include salting-out, alkali, acid and enzymatic treatment and ion-exchange chromatography. The atelocollagen market is growing exponentially, with demand in the skincare industry and for various medical applications. As a result, there is an urgent need for an eco-friendly production process with minimal manipulation. We developed a novel technique involving supercritical carbon dioxide extraction technology to remove the cells and noncollagenous substances from the porcine hide. Subsequent processes allow the production of several products, including decellularized dermal membrane, high-purity collagen particles and atelocollagen. The advantages of our process are its faster speed and lower environmental impact and its generation of multiple products, including high purity atelocollagen with complete removal of telopeptides. |
| Databáze: | Directory of Open Access Journals |
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