The in vitro Effect of Doxorubicine-G2-FA Treatment on Breast Cancer Copyright
| Autor: | Hamed Mansoor Lakooraj, Zohreh Khaki, Masoud Ghorbani, Mehdi Shafiee Ardestani, Omid Dezfoulian |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | Iranian Journal of Veterinary Medicine, Vol 14, Iss 2, Pp 147-158 (2020) |
| Druh dokumentu: | article |
| ISSN: | 2251-8894 2252-0554 |
| DOI: | 10.22059/ijvm.2019.291998.1005039 |
| Popis: | BACKGROUND: To achieve delivery of a drug to tumors, folic acid (FA) was used as a targeting ligand to change nanocarriers. Since the folate receptor has more expression in several tumor types such as breast cancer. OBJECTIVES: The present study aimed to evaluate the effect of Doxorubicine-G2-FA (Dox- G2-FA) with in vitro assays. The abbreviation of G2 represents the second generation of dendrimer synthesis. METHODS: For this purpose, Dox-G2-FA was synthesized and mass spectroscopy was used to confirm the synthesized component. Also, MTT assay, flow cytometry, and gene expression assay by real-time PCR were used to evaluate cell viability, apoptosis, and necrosis. RESULTS: In this study, the effect of Dox and Dox-G2-FA on the expression of Bax, Bcl2 genes showed that there was a significant decrease in the expression of the Bcl2 gene in the Dox-G2-FA group compared to Dox and control groups( P |
| Databáze: | Directory of Open Access Journals |
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